| Group | Club/Team Name | Team ID | Head Coach |
| Seed 1 | South Carolina United FC - Competitive SCUFC 10 Girls PRE 1 |
02208-2081G3U-0465 | Kira Campbell |
| Seed 2 | Bulls Soccer Club KEEP 2010 Bulls Girls Red |
02012-0031G3U-2592 | Stephen Roach |
| Seed 3 | Technical Soccer Club - 01 Technical Soccer Club - 13U Girls |
02226-0011G3U-8519 | Colin Widener |
| Seed 4 | United FC United FC - 2009G Elite |
01032-0011G3U-0015 | Samuel Greeley |
| Seed 5 | Augusta Arsenal Soccer club - 01 AASC 09 Girls Gold |
02224-001CG3U-0029 | Jonel Romero |
| Seed 6 | Lakeland Toros Lakelands Toros 2009 Girls |
01014-0011G3U-2057 | n/a |